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KMID : 0358920060330010077
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Journal of the Korean Academy of Pedodontics
2006 Volume.33 No. 1 p.77 ~ p.84
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Quantitative comparison of mRNA expression of glucosyltransferase(GTF) between xylitol-resistant(X^(R)) and xylitol-sensitive(X^(s)) mutans streptococci
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Lee Mi-Na
Kim Young-Jae
Lee Sung-Hoon
Kim Chong-Chul
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Abstract
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Since the long-term exposure of mutans streptococci to xylitol is known to select for xylitol-resistant(X^(R)) natural mutants, the occurrence and survival of such X^(R) strains were performed in batch culture methods.
The aim of the study was to compare the differentiation and quantification of mRNA expression of the gtf genes of X^(R) and X^(S) mutans streptococci.
Using a real-time reverse-transcription polymerase chain reaction, the expression of each gtf was determined. In X^(R) strains, the relative levels of transcription of gtfB and gtfC were decreased while that of gtfD was increased, suggesting the presence of independent promoters.
It also suggested that mutation related to production of glucosyltransferase occurred under the exposure of xylitol could explain the caries-preventive mechanisms of xylitol.
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KEYWORD
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Xylitol resistant(X^(R)), gtf, Glucosyltransferase
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